Aip2 player

Both the ethylene overproduction mutant eto1 and the constitutive ethylene response mutant ctr1 exhibit Glc Zhou et al. Gampala , Christopher D. Almost all living organisms rely on the products of plant photosynthesis for sustenance, either directly or indirectly. Sugar response pathways are integrated with other signaling pathways, such as those for light, phytohormones, stress, and nitrogen Dijkwel et al. SIS3 mediates sugar response during early seedling development.

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Interactions of the developmental regulator ABI3 with proteins identified from developing Arabidopsis seeds. The results of this experiment indicate that primers that bind to the exons on either side of the sis T-DNA insertion site fail to detect significant levels of SIS3 transcript in RNA isolated from sis Curr Opin Plant Biol 2: Please review our privacy policy.

Abscisic acid signaling in seeds and seedlings. The sis3 mutant lines display wild-type or near wild-type responses to ABA and GAs during seed germination. Cold Spring Harbor Laboratory Press. In addition, sugars, such as Suc and Glc, function as signaling molecules to regulate plant growth, development, gene expression, and metabolic processes.

Several components of plant sugar response pathways have been identified based on the conservation of sugar-sensing mechanisms among eukaryotic cells Rolland et al. Journal List Plant Physiol v. Ming-Ye Kuo is thanked for aiding in isolation of the sis mutant.

GEOSS AIP2 Disaster Management Scenario Spot Image contribution Didier Giacobbo 25 September 2008.

However, indirect effects of mutations on mRNA levels may also be informative. BMC Plant Biol 8: Search for related content. Seedlings that developed green cotyledons and true leaves were transferred to soil and grown to maturity to produce seeds.

These results suggest that any effects of Glc on SIS3 activity are at the posttranscriptional level. In the presence of 0. Trends Biochem Sci However, plauer is precedent for different sugar concentrations to have variable, and potentially opposite, effects.

A mapping population of 1, plants displaying a sis phenotype i.

SUGAR-INSENSITIVE3, a RING E3 Ligase, Is a New Player in Plant Sugar Response

To further characterize the effects of mutations in the Aip gene, quantitative real-time RT-PCR was used to compare sugar-regulated gene expression in sissisand Col-0 wild-type seeds germinating on medium containing m m Glc or m m sorbitol as an osmotic control. As the sis mutation results in complete deletion of the SIS3 gene, SIS3 transcript levels were not assayed in pllayer Data represent the means of three independent assays.

A SIS3 genomic clone 3, bpranging from bp before the start codon until the stop codon, was amplified by PCR, confirmed by DNA sequencing to be free of mutations, and cloned into the destination vector pEarleyGate Earley et al.

Seeds homozygous for the abi Laby et al.

However, linkage analysis indicated that the kanamycin-resistant phenotype conferred by the T-DNA insertion does not cosegregate with the sugar-insensitive phenotype of the sis mutant. Although predictions regarding whether SIS3 has a signal peptide or anchor are inconsistent between different software programs, all software programs examined are consistent in predicting that SIS3 has several transmembrane domains.

Carrie Readal is thanked for assistance in the early characterization of aup2 sis mutant.

T3 and T4 homozygous lines were used for complementation olayer. E3 Ubiquitin Ligases Neurobiological Mechanisms: The ABRC is thanked for providing ap2 stocks segregating for the sissisand sis mutations. Services Alert me when this article is cited Alert me if a correction is posted Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Download to citation manager Permissions.

C, Quantitative real-time RT-PCR analysis of sugar-regulated gene expression in sissisand wild-type Col-0 germinating seeds treated with m m Glc or sorbitol. Article and publication are at http: Sugar, ABA, and Paclobutrazol Assays To assay sugar response, 50 to seeds were sterilized, stratified, sown on minimal medium Kranz and Kirchheim, containing the indicated sugars, and scored for seed germination, cotyledon expansion, and true leaf formation as described Poayer et al.

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